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Journal Articles Molecular Ecology Notes Year : 2002

Seventeen new exon-primed intron-crossing polymerase chain reaction amplifiable introns in fish

Abstract

We used exon-primed, intron-crossing polymerase chain reaction (EPIC-PCR) amplification to assay variation in nuclear loci in some teleost fishes (Carangidae, Centropomidae, Chaetodontidae, Clupeidae, Holocentridae, Moronidae, Mullidae, Pomacentridae, Scombridae, Siganidae). We designed primers in the conserved regions flanking splice sites of consecutive exons of different genes, allowing the amplification of 17 putative introns. Among the satisfactory amplified systems, 14 showed length polymorphism with 2-14 alleles.
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Dates and versions

ird-03044170 , version 1 (07-12-2020)

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M. Hassan, C. Lemaire, Cécile Fauvelot, F. Bonhomme. Seventeen new exon-primed intron-crossing polymerase chain reaction amplifiable introns in fish. Molecular Ecology Notes, 2002, ⟨10.1046/j.1471-8286⟩. ⟨ird-03044170⟩
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