Isolation and characterization of microsatellites in two tropical butterflies, Drupadia theda and Arhopala epimuta (Lepidoptera: Lycaenidae) - IRD - Institut de recherche pour le développement Access content directly
Journal Articles Molecular Ecology Notes Year : 2005

Isolation and characterization of microsatellites in two tropical butterflies, Drupadia theda and Arhopala epimuta (Lepidoptera: Lycaenidae)

Abstract

Little is known about the effect of El Niño Southern Oscillation-induced fires on the genetic diversity of tropical rainforest species. Here, I report on the isolation and characterization of 10 microsatellite loci, five loci each, for two lycaenid butterfly species in East Kalimantan, Indonesia, namely Drupadia theda and Arhopala epimuta , which will be used to specifically study the impact of disturbance on genetic diversity. Microsatellite enrichment was carried out using streptavidin-coated magnetic beads. Positive colonies were identified with the three-primer polymerase chain reaction (PIMA). Cross-species amplifications conducted both within and between genera were successful in 16 out of 20 tests.
Fichier principal
Vignette du fichier
Fauvelot_2005_MEN.pdf (81.19 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

ird-03044080 , version 1 (07-12-2020)

Identifiers

Cite

Cécile Fauvelot. Isolation and characterization of microsatellites in two tropical butterflies, Drupadia theda and Arhopala epimuta (Lepidoptera: Lycaenidae). Molecular Ecology Notes, 2005, 5, pp.724 - 726. ⟨10.1111/j.1471-8286.2005.01045.x⟩. ⟨ird-03044080⟩

Collections

IRD UNC
23 View
124 Download

Altmetric

Share

Gmail Facebook X LinkedIn More