cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1. - IRD - Institut de recherche pour le développement Access content directly
Journal Articles Cellular and Molecular Biology Year : 2008

cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1.

S. Pierre
  • Function : Author
E Gouze
  • Function : Author
J Gouze
  • Function : Author
J Aubert
  • Function : Author
J-P Grillasca
  • Function : Author
Sandrine Gaillard
  • Function : Author

Abstract

The liver cDNA encoding heme oxygenase--1 (HO-1) was sequenced from European sea bass (Dicentrarchus labrax) (accession number no. EF139130). The HO-1 cDNA was 1250 bp in nucleotide length and the open reading frame encoded 277 amino acid residues. The deduced amino acid sequence of the European sea bass had 75% and 50% identity with the amino acid sequences of tetraodontiformes (Tetraodon nigroviridis and Takifugu rubripes) and human HO-1 proteins, respectively. A short hydrophobic transmembrane domain at the C--terminal region was found, and four histidine residues were highly conserved, including human his25 that is essential for HO catalytic activity. RT-PCR of mRNA from eight different European sea bass tissues revealed that, in a homeostatis state, the heme oxygenase--1 was abundant in the spleen and liver but not in the brain.
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Dates and versions

ird-01826905 , version 1 (30-06-2018)

Identifiers

  • HAL Id : ird-01826905 , version 1
  • PUBMED : 19116084

Cite

Nathalie Prévot d'Alvise, S. Pierre, E Gouze, J Gouze, J Aubert, et al.. cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1.. Cellular and Molecular Biology, 2008, pp.OL1046-54. ⟨ird-01826905⟩
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