Harbinger transposon insertion in ethylene signaling gene leads to emergence of new sexual forms in cucurbits - INRA - Institut national de la recherche agronomique Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2024

Harbinger transposon insertion in ethylene signaling gene leads to emergence of new sexual forms in cucurbits

Hadi Shirazi Parsa
  • Fonction : Auteur

Résumé

In flowering plants, the predominant sexual morph is hermaphroditism, and the emergence of unisexuality is poorly understood. Using Cucumis melo (melon) as a model system, we explore the mechanisms driving sexual forms. We identify a spontaneous mutant exhibiting a transition from bisexual to unisexual male flower, and identify the causal mutation as a Harbinger transposon impairing the expression of Ethylene Insensitive 2 ( CmEIN2 ) gene. Genetics and transcriptomic analysis reveal a dual role of CmEIN2 in both sex determination and fruit shape formation. Upon expression of CmACS11 , EIN2 is recruited to repress the expression of the carpel inhibitor, CmWIP1 . Subsequently, EIN2 is recruited to mediate stamina inhibition. Following the sex determination phase, EIN2 promotes fruit shape elongation. Genome-wide analysis reveals that Harbinger transposon mobilization is triggered by environmental cues, and integrates preferentially in active chromatin, particularly within promoter regions. Characterization of a large collection of melon germplasm points to active transpositions in the wild, compared to cultivated accessions. Our study underscores the association between chromatin dynamics and the temporal aspects of mobile genetic element insertions, providing valuable insights into plant adaptation and crop genome evolution.
Fichier principal
Vignette du fichier
Ya Huang_natureCommunication_2024.pdf (2.53 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
licence

Dates et versions

hal-04661928 , version 1 (25-07-2024)

Licence

Identifiants

Citer

Hsin-Ya Huang, Siqi Zhang, Fadi Abou Choucha, Marion Verdenaud, Feng-Quan Tan, et al.. Harbinger transposon insertion in ethylene signaling gene leads to emergence of new sexual forms in cucurbits. Nature Communications, 2024, 15 (1), pp.4877. ⟨10.1038/s41467-024-49250-9⟩. ⟨hal-04661928⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More