Anopheles squamifemur has been identified from CDC light trap collections carried out at Arca de Israel, a small community located in the extreme north-east of Bolivia (Pando Department) on the banks of the river Madera, on the border with Brazil. Anopheles costai and An. forattinii have been identified in place of An. mediopunctatus which has been removed from the Bolivian list of Anopheles species. The first identification of An. trinkae in Bolivia by Dr. J.C. Lien in 1984 is cleared. The presence of An. deaneorum in Bolivia has been confirmed by our mosquito captures carried out in Guayaramerin (Pando Department, north-east of Bolivia), a border city separated from the type locality of An. deaneorum, the Brasilian city of Guajara-Mirin, by the large Mamore River. These new findings increase to 43 the total number of known Anopheles species for Bolivia for which an updated and partially annotated checklist is given. To cite this article: F. Lardeux et al., C. R. Biologies 332 (2009).